Structures of fattiviracin family, antiviral antibiotics.

antibiotic belonging to bafilomycin, AH-1763 IIa3), a new antibiotic belonging to pluramycin group and fattiviracin Al4), a new antibiotic belonging to sugar-fatty acid lactone. Streptomyces microflavus strain No. 2445 turned out to produce at least 13 derivatives of fattiviracin (fattiviracin FV-1 to FV-13). They were isolated from culture filtrate by using column chromatographies on Diaion HP-10, silica gel, ODSand preparative HPLCwith monitoring antiviral activity. Ten derivatives except fattiviracin FV-1 to FV-3 belonged to sugar-fatty acid lactone. Fattiviracin FV-1 to FV-3 remained to be determined because of trace amount of production. As shown in Fig. 1, fattiviracins can be divided into 5 families according to the length of fatty acids moiety. Someof them will be tautomeric isomer each other. In this paper we describe the isolation, structures and biological properties of each representative from 5 families such as FV-4 (1), FV-8 (2)5), FV-9 (3), FV-10 (4) and FV-13 (5). A slant culture of the strain No. 2445 was inoculated into 200 ml Erlenmeyer flask containing 50 ml of seed medium which consisted of glucose 2.0%, starch 3.0%, corn steep liquor 1.0%, soybean flour 1.0%, peptone 0.5%, NaCl